Enzyme-Linked Immunosorbent Assay (ELISA) is a test designed for detecting and quantifying substances such as antibodies, proteins, and hormones in a sample. Antibodies are proteins produced by the body’s immune system when it detects harmful substances, called antigens. The enzyme-linked immunosorbent assay ( ELISA ) (/ ɪˈlaɪzə /, / ˌiːˈlaɪzə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. [1] . ELISA stands for Enzyme-Linked Immunosorbent Assay . ELISA is widely used to detect diseases such as HIV/AIDS, hepatitis, dengue fever, and COVID-19. The Enzyme-Linked Immunosorbent Assay (ELISA) operates on the concept of antigen-antibody binding. What is an ELISA test? ELISA is an abbreviation for " enzyme-linked immunosorbent assay ." In 1974, P. Perlmann and E. Engvall developed the test as a substitute for certain radioimmunoassay tests, and eventually, it replaced the western blot test for HIV confirmation.
