Tissue culture is a method for growing or maintaining tissue in vitro under controlled conditions. The tissue is taken directly from an organism for culture without first dissociating cells. The ... Learn what tissue culture is in biology, how it works, and why it is important for plants and animals. Explore the different types, steps, and applications of tissue culture in agriculture, medicine, and conservation. Tissue culture is defined as a technique used to grow cells, tissues, or organs in a controlled, sterile environment. It allows scientists to produce thousands of identical plants from a single parent plant, making it a powerful tool in plant propagation. This method is especially useful in agriculture as part of strategies for enhancement in food production, helping in the rapid multiplication of high-yielding and disease-resistant crops. Plant tissue culture is the in-vitro aseptic culture of cells, tissues, or whole plants under controlled nutritional and environmental conditions, often to produce clones of plants.