Gram staining: Gram stain is a technique to

Gram stain is a technique to impart color to the bacterial cell to differentiate between gram -positive bacteria and gram -negative bacteria based on cell wall composition. Gram Staining is a laboratory procedure that consists of four reagents crystal violet (primary stain ), iodine (mordant), decolorizer (ethyl alcohol), and safranin (counter stain ) to stain the bacterial cell. Hans Christian Gram is a Danish bacteriologist who named this stain and developed this method in 1884. The basic ... Gram staining is the differential staining that is used to differentiate the bacteria majorly into two groups, i.e. gram -positive and gram -negative based on the cell-wall difference and by the sequential application of crystal violet, iodine, alcohol and safranin. Gram stain is a technique to classify bacteria into gram -positive and gram -negative based on their cell wall properties. Learn how it works, who invented it and when it is used in medicine and research. Gram staining is a cornerstone technique in microbiology, crucial for differentiating between two major groups of bacteria based on their cell wall properties. Developed by Hans Christian Gram in 1882, this staining method has become a standardized protocol used in laboratories worldwide. In this comprehensive guide, we delve into the intricate details of the Gram staining protocol, exploring its principle, procedure, and significance in microbiological diagnostics. What is a Gram Stain ...