Introduction High-performance liquid chromatography ( HPLC ) is an indispensable analytical technique in the pharmaceutical industry. It allows for the separation, identification, and quantification of the chemical components of pharmaceutical drug products and drug substances. With its high sensitivity, accuracy, and reproducibility, HPLC enables pharmaceutical companies to analyze everything from drug raw materials, intermediates, and final products to perform quality control, optimize ... HPLC is a technique for separating and detecting compounds dissolved in a liquid sample using a column and a detector. Learn about the HPLC apparatus, separation, and chromatogram in this web page. What is HPLC in chemistry? HPLC stands for High-Performance Liquid Chromatography, a highly effective technique used for separating, identifying, and quantifying chemical compounds in complex mixtures. It is a cornerstone of analytical chemistry and plays a pivotal role in pharmaceuticals, food analysis, environmental monitoring, and biochemical research. In this article, we will cover what is HPLC , its full form , principle, instrumentation, the purpose of an HPLC test, how to interpret an ... What is High-Performance Liquid Chromatography ( HPLC )? HPLC is used to separate, identify, and quantify compounds in a mixture that are dissolved in a solution. An HPLC separation involves the injection of a small volume of liquid sample into an HPLC column packed with particles (typically 3 to 5 microns in diameter) called the stationary phase.
